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Page number:198 
Remarks (internal):Species description centered upon NRRL 2058 received in May 1946, from Professor Weston, Harvard University, as a strain isolated from ex-posed textiles in Florida. Represented also by NRRL 2059 received in April 1944, from Laura A. Kolk, Brooklyn College, Brooklyn, New York, as a highly pigmented culture from a dilution plate of a water sample taken from a small pond. Also represented by NRRL 2060, received in May 1945 from Dr. Lawrence White, Philadelphia Quartermaster Depot, as a strain isolated in Florida from exposed cellulose-nitrate covered cellophane. When first received, this latter strain was diagnosed as Penicillium implicatum Biourge var. aureo-marginatum Thom. More careful examination of the strain indicates its proper placement to be with P. multicolor G.-M. and P.
Penicillium multicolor was isolated originally from Russian soil and was successfully cultivated upon a variety of substrata including potato, carrot, and beet. It did not coagulate milk, thrived in media containing 2 to 3 percent lactic acid, but grew poorly in alkaline media. It was characterized particularly by its pigmentation which varied with the sub-stratum and ranged from yellow-orange to dark red, with pigment "grains" often appearing in the large hyphae. The penicilli were monoverticillate and usually unbranched.
Attempts to obtain the type culture failed. An organism bearing this name received from the authors was found to be replaced by another mold, or to have lost the characteristic features which distinguished it when first isolated. A form believed to approximate the type was subsequently sent to Thom by Professor Waksman as an isolate from New Jersey soil, but this was quickly lost from the collection. Another strain discussed by Thom in his Monograph (1930, p. 213) and in the laboratory was referred to as the "paint" organism, but under continued artificial cultivation lost its pigment producing power. Other strains suggestive of the Penicillium multicolor description were isolated from American sources but failed to survive for long periods in culture. Cultures of this type are apparently not especially common in nature, but they have been encountered from time to time among soil isolates in this laboratory and have appeared among the strains isolated from deteriorating fabrics and other military equipment exposed in tropical and subtropical areas.
While we cannot assume with certainty that the highly colorful strains we assign to Penicillium multicolor G.-M. and P. duplicate in all details the strain originally examined by the authors of this species, there is considerable reason for believing that they must have based their species upon a monoverticillate form similar to those considered above.
 
Description type:Non-original description 
Description:Penicillium multicolor Grigorieva-Manoilova and Poradielova, in Archives des Sciences Biologiques Leningrad 19: 117-131, fig. 1 and one plate with photographs 1-6. 1915 (in Russian). Thom, The Penicillia, pp. 212-213. 1930.
Colonies on Czapek's solution agar growing rather restrictedly, about 2.0 to 2.5 cm. in 8 to 10 days at room temperature, radially furrowed, consisting of a comparatively thick felt 1.0 to 1.5 mm. deep (fig. 54A and D), with basal portion close-textured and fairly tough, with surface loose-textured, strictly velvety and heavy-sporing in some strains, in others light sporing and almost floccose but presenting a velvety appearance, or showing both in the same culture, with growing margin 1 to 2 mm. wide, pale yellow to rich orange in color, conidial areas developing in localized central to sub-central patches against a background of yellow to orange or orange-red vegetative mycelium (appearing studded with orange to orange-red granules when viewed under low magnification) in some strains, in others heavily sporing throughout with the massed conidial structures characterizing the colony, in blue-green shades near greenish glaucous blue to deep bluish gray-green (Ridgway, Pl. XLII); exudate limited to abundant, yellow to pale orange; odor not pronounced, suggesting mushrooms; reverse in bright orange-red shades near flame scarlet or mars orange to burnt sienna (R., Pl. II); conidiophores abundantly produced in some strains, sparingly in others, arising mostly from the substratum (fig. 54E) but sometimes from aerial hyphae, usually unbranched, varying in length from comparatively short up to 300 to 400 µm by 2.0 to 2.5 µm, apparently smooth-walled in some strains, granular in others, or showing both conditions in the same strain, walls sometimes studded with orange colored crystals when viewed dry, apices slightly enlarged or in some strains definitely vesicular up to 5.0 or 5.5 µm; penicilli strictly monoverticillate consisting of compact terminal clusters of sterigmata, usually 6 to 10 or 12 in the verticil (fig. 54F), in some strains more, mostly 8 to l0 µm by 2.0 µm with apices parallel or divergent, bearing chains of conidia up to 100 µm long, loosely parallel but not producing definite columns; conidia globose to subglobose, about 2.0 to 2.5 µm. in diameter with walls appearing finely roughened.
Colonies on steep agar growing more rapidly than on Czapek, up to 4.5 to 5.0 cm. in 10 to 12 days, radially furrowed (fig. 54B), texture as described above, with margin usually broad, yellow to orange-red in color, heavier sporing but in the same tints as on Czapek; exudate limited; odor not pronounced; penicilli as described above but with conidial chains longer, often exceeding 100 µm.
Colonies on malt agar 3.0 to 4.0 cm. in 10 days, plane, appearing velvety commonly tufted in central colony areas (fig. 54C), in mixed red (from heavily encrusted vegetative hyphae) and blue-green (from conidial structures) shades, with the former predominating in some strains, the latter in others; exudate limited; odor not pronounced; reverse in bright orange-red shades near grenadine red or flame scarlet (R., Pl. II); conidiophores arising almost entirely from the substratum, bearing penicilli as described above but with conidial chains up to 150 µm in length.
 
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