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 Add this item to the list   Phanerochaete chrysosporium
   
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Page number:127 
Description type:Culture description 
Description:Phanerochaete chrysosporium
Growth Characters: Radial growth on 1.5t Difco Bacto Malt Extract Agar 45-60 mm in three days at 25C; mat (Fig. 5) very thin, white, appressed radiating, some isolates with granular surface 1-2 cm behind the advancing margin, others with granular surface after margin reaches edge of plate, granular aspect spreading from inoculum until entire plate is covered; agar bleached below inoculum and often over whole plate; some isolates producing creamcolored, effused hymenial surface, usually in 18-28 days, forming all structures typical of fruiting in nature; other isolates never fruit. On gallic acid agar (Davidson, et al. 1938) growth 24-30 mm diam/week, mat thin-woolly, margin irregular, agar not stained (Fig. 6); on tannic acid agar (Davidson, et al. 1938) no growth or staining of agar (Fig. 7); tests with syringaldazine and gum guaiac (Harkin and Obst 1973) negative, indicating lack of laccase, peroxidase and tyrosinase.
Microscopic Characters: Hyphae of margin hyaline, thin walled, 3.5-7 µm diam, smooth, infrequently branched, densely stained in phloxine, first septum usually occurring more than 1 mm from tip, clamps lacking.
Aerial hyphae (Fig. 8) 2-4 vm diam, hyaline, thin walled, smooth or occasionally encrusted with long narrow crystals, septate, lacking clamps, branched frequently, these branches often developing as conidiophores; submerged hyphae (Fig. 9) (2.5-) 3.5-6 (-8) vm diam, mostly thick walled, occasionally thin walled in narrower hyphae, hyaline, smooth, septate, lacking clamps, frequently branched, usually at nearly right angles.
Conidia only on aerial hyphae; terminal persistent conidia (aleuriospores of Carmichael, 1962) (Fig. 10) 5-lO x 3-6 µm, ovate to elliptical, truncate at attachment to poorly differentiated conidiophore, smooth, hyaline, densely granular, sometimes guttulate, with slight wall thickening, not reacting with Melzer's reagent; arthric, retrogressive, fragmenting conidia (arthrospores of Carmichael, 1962) (Fig. 11) variable in size and shape (cylindrical, ovoid, clavate, branched, or fusoid), hyaline, densely granular, with slightly thickened walls, not reacting with Melzer's reagent; a third type of conidia (chlamydospores of Carmichael, 1962) (Fig. 12) terminal or intercalary, pyriform, spherical, to truncate-fusiform, hyaline, smooth, with densely granular contents and thick walls, up to 60 µm in longest dimension.
Temperature Relations: Average growth rates (mm radius/day) for the temperatures indicated are: 12C, 2-3 mm; 16C, 3-5 mm; 20C, 9-13 mm; 24C, 14-19 mm; 28C, 18-24 mm; 32C, 26-32 mm; 36C, 30-36 mm; 40C, 35-42 mm; 44C, 28-33 mm; 50C, 0-6 mm. According to the method of Davidson, et al. (1942) the key pattern would be A-0-F 2-3-4-10-14 or B-O-F 2-3-4-5-6-10-14-16. Using the method of Nobles (1965) the following species code is obtained: 1.6.14.24. 32.33.34.35.36.40.41.(48).54.55.
 
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