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 Add this item to the list   H. alpestre (GB 392, GB 407):
   
Literature:
 
Page number:186 
Description type:Culture description 
Description:H. alpestre (GB 392, GB 407):
Mat cottony, floccose - granular, with drop-like exudates; hyphae with clamps, ordinarily branched, straight, 1,5-8 µm wide, thin-walled; gloeoplerous hyphae present, with clamps in the connection with generative hyphae, otherwise simpleseptate, sometimes appearing as gloeocystidium-like structures, with an oily, yellowish, sulfo-negative content; lemonshaped chlamydospores present, 6-8x9-11 µm, intercalary formed.
After 3 weeks growth fructification was observed (GB 407). Fig. 2 B, C, 3 D.
Code: 2a. (21b). 3c. 26. 34. 36. (37). 38. 43. 48. 55. 60. 61.
Oxidase reactions:
Syringaldazine + 1-Naphtol +
Gum Guaiac + Guaiacol +
p-Cresol + L-Tyrosine -
Polarity. GB 392 - A1 B1: 1; A2B2: 4; A2 B1: 2,3.
Cytology: Monosporous mycelia with uninucleate cells, clamped mycelia dicaryotic. Chlamydospores uninucleate when formed on haploid mycelium, binucleate when formed on clamped mycelium.
The culture studies reveal additional separating characters between the two species. In H. coralloides there is a slow
growth, mat is plumose and reaction with p-cresol is negative.
In H. alpestre there is a rapid growth, mat is cottony, floccose, presence of chlamydospores and a positive reaction with p-cresol.
Single spore cultures from the following specimens were used in compatibility tests:
Hericium coralloides/ GB 145/ Denmark, Mön/ 1980-09-18--21/ N. Hallenberg.
Di-mon matings gave the following results (dicaryotic x monocaryotic mycelia):
LY 9910 x GB 392/1,4 = - LY 9910 x GB 145/3 = +
LY 9910 x GB 407/1. = - LY 9910 x LY 9923/1,2 = +
LY 9910 x GB 331/1 = - GB 166 x GB 145/1 = + LY 9910 x GB 145/1 = -
From above it is obvious that H. coralloides is not compatible with H. alpestre Further, within H. coralloides partial compatibility is observed between GB 145 (Denmark) and LY 9910 (France) and its progeny (GB 820). Even in the combination between GB 145/3 and LY 9910 which resulted in the formation of clamps, the dicaryotization was very slow and incomplete.
Both Boidin (1958) and Stalpers (1978) described the culture characters of H. coralloides (cultures from CBS). From their descriptions, however, it is obvious that they referred to what here is called H. alpestre.
 
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