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 Add this item to the list  Poria andersonii
Page number:163 
Description type:Culture description 
Description:Poria andersonii
Petri-dish cultures: Growth slow forming in 7 days a mat 2-3 cm. in diameter. Mat either cottony or silky-cottony, thin, and not raised above the agar or fine woolly to felty, raised and compacted, primuline yellow, mustard yellow and apricot yellow to as dark as antimony-yellow and yellow-ochre.
In 14 days mat 4.5-7 cm. in diameter (Fig. 2, A, B), very variable, usually raised, compacted, with definite concentric rings, often showing pronounced segments, mostly felty but occasionally long cottony about inoculum; light-orange-yellow to yellow-ochre, raw sienna and buckthorn-brown; margin either rising abruptly from the agar or with a thin, appressed, yellowish or almost colorless zone of advancing growth. Marginal characters very variable as some cultures may show thin marginal growth and dark colored staled areas over all or only a portion of the surface.
Hyphae staining with eosin, 2-5 µm in diameter (Fig. 1, A), no clamps; from staled areas under center, yellowish, collapsed, non-staining, with numerous cross-walls and often broken up into irregular lengths; fibrous hyphae thick-walled, dark brown, 2-4(-5) µm in diameter (Fig 1, B); long dark-brown setal hyphae common in some cultures, rare in others, up to 8 µm in diameter and 250 µm long (Fig. 1, C); bulbous setae common in compacted, staled areas and in fruiting structures (Fig. 1, D); basidiospores occasionally formed in poroid areas, ellipsoid, smooth, yellowish-green, 5-8 X 4-5 µm (Fig. 1, E).
Temperature relations: Optimum temperature for growth approximately 35° C. Average diameter of mats in 7 days at constant temperatures,5 in dark, as follows: 1.0 cm., 20°; 3.1 cm., 25°; 5.2 cm., 30°; 5.5 cm., 35°; slight growth, 40°.
Test-tube cultures: In 14 days mat raised, fine woolly to felty, or appressed-felty, mustard-yellow and apricot-yellow to as dark as antimony-yellow and yellow-ochre.
In 28 days mat on slant compacted woolly to felty, yellow-ochre to buckthorn-brown and raw sienna; on agar cylinder lighter, mustard-yellow to antimony-yellow and yellow-ochre, often with pronounced zones, tough, forming a heavy film which is difficult to tear with a needle. An occasional isolate will form well-developed pores on a raised mycelial cushion at the lower end of the slant.
Flask cultures: The mycelium, on maple and oak blocks, is at first yellow, cottony, becoming in 10 months compacted ochraceous buff, cinnamon or yellow-ochre to as dark as buckthorn-brown or ochraceous tawny; irregular areas outlined by narrow black lines rather common. Sporophores are formed in some flasks, usually where the mycelium makes contact with the glass. The sporophores are much distorted by pressure against the glass and produce basidiospores in sufficient abundance as to cause a yellowish-green spore print. Wood decayed by P. Andersonii becomes very soft, white and spongy.
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