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 Add this item to the list   Neotypification of Boletus pini.-
Page number:841 
Remarks (public):The neotype and paraneotypes were all collected within a few kilometers of each other, south of Lisbon. Brotero's (1804) description does contain some discordant elements with regard to the neotype, i.e. "pulvinatus" (cushion shaped) and "subtus lateritius" (brick-colored below). However, if the ontogeny of basidiomata for this species is considered, we can envision a somewhat cushion-shaped basidioma in early stages of development (1-2 yr), accompanied by an undersurface that at some point in its annual development would be dull reddish-brown (perhaps near dull brick-colored).
There is a specimen that is a potential candidate for neotype in the herbarium of Pereira Coutinho (Flora Lusitanica) no. 6371 (LISU), labelled as Trametes pini (Thore) Fr. and inscribed "Nos Pinheiros: Pinhal de Leiria". Microscopically, this specimen is quite similar to the neotype. Macroscopically it is ungulate to pulvinate (now divided into several mounted pieces) . However, it was deemed important to select neotypic material from which cultures have been obtained, thus providing a basis for more detailed comparisons, at the molecular and physiological levels, between geographically separated populations of the Phellinus pini-complex.
Description type:- 
Description:Neotypification of Boletus pini.-
Phellinus pini (Brot.: Fr.) Ames, Ann. Mycol. 11:246. 1913. Figs. 1-6
Boletus pini Brot., Flora Lusitanica 2:468. 1804.
Daedalea pini (Brot.: Fr.) Fr., Syst. Mycol. 1:336. 1821.
Neotype. Portugal. Estremadura, Almada, Fonte da Telha, on live Pinus pinaster Ait., 19 Jan. 1994, coll. I. Melo and J. Cardoso, no. 6170 (LISU).
Paraneotypes. Portugal. Estremadura, Palmela, PoceirÀ£o, on live standing P. pinaster, no. 6172; , Sesimbra, MaçÀ£, no. 6171 and Barreiro, Penalva, no. 6174, coll. I. Melo and J. Cardoso, 19 Jan. I994 (LISU).
Cultures. Isolates obtained from the context of the neotype and paraneotypes are stored at CFMR and ATCC.
Basidioma 12 x 7 x 5 cm, sessile, pileate, usually solitary, woody, becoming perennial. Upper surface at first ferruginous brown (near 7.5YR 5/2) and hirsute, then with a black pellicle up to 1.5 mm thick, radially and concentrically cracked, sulcate, overlain by a uniformly dull green effuse, lichenized discomycetous growth. Margin ferruginous brown (near 7.5YR 4/4), acute to somewhat rounded, 3-4 mm wide. Pore surface dull yellowish-brown with a faint citrine cast (near 2.5Y 4/4). Pores 1-2(-3) per mm, round to mostly angular with some elongated radially. Tubes indistinctly stratified, individual strata up to 9 mm thick, individual tubes filled with brown hyphae (more rarely white). Context up to 2 mm thick, concolorous with the margin and somewhat darker than tramal tissues, appearing distinctly radially oriented when the surface pellicle is removed. Contextual skeletal hyphae (3.5-) 4-5 µm diam, becoming thickwalled to solid, septa absent or with some adventitious septa present, unbranched, dull brown. Contextual generative hyphae of two kinds, some 2-2.5 µm, thin-walled, septate, branching frequently; others 3.5-5 µm diam, thin-walled but some becoming sclerified, septate, branching infrequently, pale brown. Tramal skeletal hyphae 3-4 µm diam, aseptate, thick-walled to solid, dull brown, interwoven to somewhat parallel. Tramal generative hyphae 1-2 µm diam, septate, thin-walled, frequently branched, hyaline. Tramal setal hyphae infrequent, 70-75 x 10-12.5 µm, thick-walled, dark ferruginous brown. Hymenial setae (30-)40-50(-60) x 15-30(-40) µm, form highly variable from subulate, ventricose, to rostrate, dull ferruginous brown, thick-walled. Basidia 8-10 x 5-6 µm, broadly clavate to obovate, basidial honeycomb structure absent from the hymenium. Basidiospores 56(-6.5) x 4-5(-5.5) µm, mostly broadly ellipsoid to sometimes subglobose, often tapering towards the apiculum and flattened adaxially, some with thickened walls, hyaline in 5% KOH and H20 (a few pale rusty brown spores present), negative in Melzer's reagent, contents and walls cyanophilous.

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