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Page number:199 
Description type:Culture description 
Description:Rigidoporus microporus (Fr.) Overeem Fig. 6 Icon. Fung. Malayeanum, 1. 197A.
Culture studied: cc 797 (=_ FLOR 11353; BRAZIL, SC, llha de Santa Catarina, Canto dos Araças; Althoff, Gerber & Neves; 22/3ü/1995).
Macroscopic characters: Mat hyaline, appressed without differentiation, excepting few little cottony tufts by the dish margin. Margin irregular; growth very rapid, dish covered by the first week. Reverse unchanged. Odor musty.
Microscopic characters: Mycelium formed by generative, simple-septate, thin or thickwalled hyphae (1,5 - 6,0 µm diam.). Fiber hyphae absent. Pyramidal (3,0 - 5,5 µm diam.) or irregular crystals present in the center of the mat, the last ones free or incrusted on generative hyphae, seen after the 3th week. Cottony tufts at the margin composed of generative, simple-septate hyphae and few hyphae with clamps on a few of the septa (1,5 - 4,5 µm) seen only at last week. Sexual structures absent.
Enzymatic tests: GAA: +++; TAA: +++; Laccase: ++(a-naphtol), -(guaiacol), +++(syrlngaldazine); Cytochromo oxidase: +(tmpda); Peroxidase: ++(pyrogallol); Tyrosinase:-(p-cresol)
Species code: 2. 5. 7. 32. 36. 38. 51. 54.
Remarks: This genus is characterized in the carpophore by simple-septate hyphae. R. microporus does not have cystidia and spore size is 3.5 - 5.0 µm long and diffus from R. lineatus (Pers.) Ryv. that has cystidia and bigger spores (4.5 - 6.0 µm long), both species are pileate. R. vinctus (Berk.) Ryv. is resupinate, does not have cystidia and the spores has an intermediate size (4.0 - 5.5 µm long). R. microporus was studied in culture before by Bakshi et ai. (1963) and Wright and Deschamps (1975) without the mention of clamped hyphae. In our study, a eonspicuous characteristic in the culture was the presence of simple-septate and clamped hyphae together. Studies with R. lineatus (Bakshi et al., op. cit.; Neves, 1996) and with R. vinctus (Hood & Dick, 1988) showed clamped hyphae in culture. Thus, clamped hyphae may be a useful taxonomy feature in Rigidoporus cultures.
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