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 Add this item to the list  Malassezia globosa
   
ITS Type to Centrality distance:93.5532 
Assimilations:
Glucose ?
Inulin ?
Sucrose ?
Raffinose ?
Melibiose ?
Galactose ?
Lactose ?
Trehalose ?
Maltose ?
Melezitose ?
Methyl-α-D-glucoside ?
Soluble starch ?
Cellobiose ?
Salicin ?
L-Sorbose ?
L-Rhamnose ?
D-Xylose ?
L-Arabinose ?
D-Arabinose ?
D-Ribose ?
Methanol ?
Ethanol ?
Glycerol ?
Erythritol ?
Ribitol ?
Galactitol ?
D-Mannitol ?
D-Glucitol ?
myo-Inositol ?
 
DL-Lactate ?
Succinate ?
Citrate ?
D-Gluconate ?
D-Glucosamine ?
N-Acetyl-D-glucosamine ?
Hexadecane ?
Nitrate ?
Nitrite ?
Vitamin-free ?
2-Keto-D-gluconate ?
5-Keto-D-gluconate ?
Saccharate ?
Xylitol ?
L-Arabinitol ?
Arbutin ?
Propane 1,2 diol ?
Butane 2,3 diol ?
Cadaverine ?
Creatinine ?
L-Lysine ?
Ethylamine ?
50% Glucose ?
10% NaCl/5% glucose ?
Starch formation ?
Urease ?
Gelatin liquefaction ?
Cycloheximide 0.01% ?
Cycloheximide 0.1% ?
 
Number of ascospores per ascus:? 
Reddish diffusing pigment:? 
Colony margin:? 
Filaments:? 
Asci evanescence:? 
Asci shape:? 
Ascospores with gelatinous sheath:? 
Ascospores with a groove:? 
Basidia septation:? 
Basidia shape:? 
Basidia catenate-solitary:? 
Teliospores:? 
D-Glucose (F1):? 
D-Galactose (F2):? 
Maltose (F3):? 
Me a-D-Glucoside (F4):? 
Sucrose (F5):? 
a,a-Trehalose (F6):? 
Melibiose (F7):? 
Lactose (F8):? 
Cellobiose (F9):? 
Melezitose (F10):? 
Raffinose (F11):? 
Inulin (F12):? 
Starch (F13):? 
D-Xylose (F14):? 
Growth on YM/malt agar:After 7 days at
32C, single colonies are raised, folded to cerebriform, 34 mm in
diameter, rough and brittle, pale yellowish, shiny or dull, and with
the margin slightly lobate (Fig. 148.19). Cells are spherical, 2.58 μm
in diameter, and budding is monopolar on a rather narrow base
(Fig. 148.20). Short filaments, reminiscent of germinative tubes of
Candida albicans, may be present, particularly in primary cultures
(Fig. 148.21). A precipitate is produced on modified Dixon's agar, but
not on regular Dixon's agar.
 
Year:1996 
Synonyms:?Pityrosporum orbiculare Gordon (1951) (no extant culture, but the
morphological description suggests that this may be a synonym)
Malassezia furfur serotype B (Cunningham, Leeming, Ingham &
Gowland 1990)
Malassezia furfur sensu Midgley (Midgley, 1993)
 
Authors:Midgley, Guého & Guillot 
Sexual reproduction:? 
Colony colour:
white ?
cream ?
yellowish ?
orange ?
 
pink ?
red ?
buff ?
dark brown to black ?
 
Colony appearance:
arachnoid ?
farinose ?
smooth ?
 
venose ?
warty ?
furrowed ?
 
Colony texture:
fluid ?
mucoid ?
 
butyrous ?
membranous ?
 
Asexual reproduction:
fission ?
budding ?
multilateral budding ?
bipolar budding ?
monopolar budding ?
sympodial budding ?
annellidic (percurrent) budding ?
 
on sterigma ?
on denticles ?
arthroconidia ?
chlamydospores ?
endospores ?
symmetrical ballistoconidia ?
asymmetrical ballistoconidia ?
 
Cell shape:
lemon ?
triangular ?
lunate ?
allantoid ?
dumb-bell shaped ?
 
round ?
oval ?
cylindrical ?
fusiform ?
   
 
Ascospores shape:
hat shaped ?
cap shaped ?
saturn shaped ?
walnut shaped ?
conical ?
oblong ?
round ?
 
oval ?
smooth ?
rough ?
reniform ?
allantoid ?
needle shaped ?
whip like ?
 
Conjugation:
cell with cell ?
cell with its bud ?
 
clamp connexions ?
pseudoclamp connexions ?
 
Coenzyme Q system:0 
Link to MycoBank:
 
Yeasts physiological data:
C1 D-Glucose ?
C2 D-Galactose ?
C3 L-Sorbose ?
C4 D-Glucosamine ?
C5 D-Ribose ?
C6 D-Xylose ?
C7 L-Arabinose ?
C8 D-Arabinose ?
C9 L-Rhamnose ?
C10 Sucrose ?
C11 Maltose ?
C12 a,a-Trehalose ?
C13 Me a-D-Glucoside ?
C14 Cellobiose ?
C15 Salicin ?
C16 Arbutin ?
C17 Melibiose ?
C18 Lactose ?
C19 Raffinose ?
C20 Melezitose ?
C21 Inulin ?
C22 Starch ?
C23 Glycerol ?
C24 Erythritol ?
C25 Ribitol ?
C26 Xylitol ?
C27 L-Arabinitol ?
C28 D-Glucitol ?
C29 D-Mannitol ?
C30 Galactitol ?
C31 myo-Inositol ?
C32 D-Glucono-1,5-lactone ?
C33 2-Keto-D-Gluconate ?
C34 5-Keto-D-Gluconate ?
C35 D-Gluconate ?
C36 D-Glucuronate ?
C37 D-Galacturonate ?
C38 DL-Lactate ?
C39 Succinate ?
C40 Citrate ?
C43 Propane 1,2 diol ?
C44 Butane 2,3 diol ?
C45 Quinic acid ?
C46 D-glucarate/Saccharate ?
C47 D-Galactonate ?
C48 Palatinose ?
C49 Levulinate ?
C50 L-Malic acid ?
 
C51 L-Tartaric acid ?
C52 D-Tartaric acid ?
C53 meso-Tartaric acid ?
C54 Galactaric acid ?
C55 Uric acid ?
C56 Gentobiose ?
C57 Ethylene glycol ?
C58 Tween 40 ?
C59 Tween 60 ?
C60 Tween 80 ?
N1 Nitrate ?
N2 Nitrite ?
N3 Ethylamine ?
N4 L-Lysine ?
N5 Cadaverine ?
N6 Creatine ?
N7 Creatinine ?
N8 Glucosamine ?
N9 Imidazole ?
N10 D-Tryptophan ?
N11 D-Proline ?
N12 Putrescine ?
V1 w/o vitamins ?
V2 w/o myo-Inositol ?
V3 w/o Pantothenate ?
V4 w/o Biotin ?
V5 w/o Thiamin ?
V6 w/o Biotin & Thiamin ?
V7 w/o Pyridoxine ?
V8 w/o Pyridoxine & Thiamin ?
V9 w/o Niacin ?
V10 w/o PABA ?
O1 Cycloheximide 0.01% ?
O2 Cycloheximide 0.1% ?
O3 Acetic acid 1% ?
O6 10% NaCl ?
O7 16% NaCl ?
O8 Growth at pH=3 ?
O9 Growth at pH=9.5 ?
O10 Fluconazole ?
? ?
? ?
? ?
? ?
? ?
? ?
? ?
? ?
 
rDNA sequences 26S:
 
rDNA sequences ITS:
 
Temperatures growths tests :
at 4ºC ?
at 12ºC ?
at 15ºC ?
at 19ºC ?
at 21ºC ?
at 25ºC ?
 
at 30ºC ?
at 35ºC ?
at 37ºC ?
at 40ºC ?
at 42ºC ?
at 45ºC ?
 
Molecular % G+C (Average):? 
Gene sequence accession numbers, type strain:D1/D2 LSU
rRNA5AY387228, ITS5AY387132.
 
Origin of the strains studied:CBS 7966 (ATCC 96807, GM35), isolated
from pityriasis versicolor, UK; CBS 7874, from dandruff, UK;
CBS 7986, from a patient with seborrheic dermatitis; CBS 7990, from
healthy human skin; CBS 8744, from scalp; CBS 8745, from pityriasis
versicolor on the upper chest of a man.
 
Type strain:CBS 7966 
Ecology:Malassezia globosa is known from healthy and diseased
human skin (Aspiroz et al. 1999, 2002, Crespo Erchiga and Delgado
Florencio 2006, Crespo Erchiga and Guého 2005, Crespo Erchiga et al.
2000, Gemmer et al. 2002, Guého et al. 1996, Gupta et al. 2004a,b)
and from animal skin, e.g., cats, horses and domestic ruminants, and
ears of healthy bovines (Bond et al. 1997, Crespo et al. 2002, Duarte
et al. 1999). The species has been reported to occur at high frequency
in healthy as well as diseased bovines with otitis in Brazil (Duarte
et al. 1999), with the latter disease known to be associated with
nematodes (Duarte et al. 2001). DNA of M. globosa has also been
detected in European soil forest nematodes of the genus Malenchus
(Renker et al. 2003), thus suggesting that the occurrence of the species
may not be limited to warm-blooded animals. Even more surprising
was the detection of DNA of the species in soils from the
Antarctic Dry Valleys. The authors postulated that the occurrence of
the yeasts may be associated with nematodes, which are prevalent in
Dry Valley soils (Fell et al. 2006). The possible interactions between
nematodes and M. globosa need further study to determine whether
nematodes represent a natural reservoir of the species.
 
Clinical importance:Malassezia globosa seems to be the most
important species involved in pityriasis versicolor, atopic dermatitis,
and seborrheic dermatitis and dandruff (Crespo Erchiga and
Guého 2005, Crespo Erchiga et al. 1999, 2000, Gemmer et al. 2002,
Guého et al. 1996, Gupta et al. 2001, 2004b, Nakabayashi et al. 2000).
The species is most commonly isolated from lesions of pityriasis
versicolor (Aspiroz et al. 2002, Crespo Erchiga et al. 1999). The species
is isolated from seborrheic dermatitis with the same frequency
from both lesional and non-lesional skin (Nakabayashi et al. 2000).
In seborrheic dermatitis/dandruff patients, the species is commonly
isolated in conjunction with M. restricta. M. globosa is isolated predominantly
with M. restricta from atopic dermatitis patients, and
these two species account for 30.334.7% and 45.451.0% of all
Malassezia species isolated, respectively (Sugita et al. 2006). It is
likely that these diseases are caused by a convergence of
M. restricta and M. globosa, in combination with increased host
response to the fungi or their metabolites. Gaitanis and collaborators
detected and identified only M. globosa from pityriasis versicolor
using a DNA-PCR procedure directly applicable to skin scales
(Gaitanis et al. 2002). In animals, the species is clinically important,
as it was the predominant species in animals with otitis (Duarte
et al. 1999). With the rDNA sequences of the InterGenic Spacer
1 (IGS1), Sugita et al. (2003a) characterized four genotypes within
the species having short sequence repeats CT(n)2x and GT(n)2x.
These authors suggested that one of these genotypes may be related
to atopic dermatitis.
 
Additional comments:Malassezia globosa corresponds to the formerly
recognized M. furfur serovar B (Cunningham et al. 1990). In
contrast to M. furfur, M. globosa has a stable micromorphology with
spherical cells. The species does not grow with natural virgin oil or
oleic acid as unique lipid supplementation or with any individual
lipid supplements and, unlike M. obtusa, does not split esculin. This
species requires complex media such as Dixon, modified Dixon or
LNA. However, it can be easily recognized morphologically with its
cerebriform colonies and spherical cells.
Similarly to other Malassezia species, M. globosa displays a
peculiar cell-wall ultrastructure (Fig. 148.23). The initial structure
with electron-dense layers, interrupted by a helicoidal translucent
band, is associated with a secondary groove system and electronlucent
bands disposed at more or less right angles between the
helicoidal system. This double system has been visualized by the
freeze-fracture replication technique (Breathnach et al. 1976), and
by tangential sectioning of the cell walls using TEM (E. Guého and
H. Mittag, unpublished observations). The cells of the species are
difficult to lyse, probably owing to the peculiar cell-wall
ultrastructure.
 
Systematics:Malassezia globosa is related to M. restricta based on
nucleotide sequences of the D1/D2 domains of the LSU rRNA gene
(Gupta et al. 2004b, Sugita et al. 2002) (Fig. 148.1), but a significant
genetic distance occurs between the two species (6.1% nucleotide
divergence). M. pachydermatis forms a weakly supported basal lineage
to both these species. LSU rRNA gene D1/D2, ITS and IGS1
sequences suggest that some genetic heterogeneity occurs within
the species (Gupta et al. 2004b, Sugita et al. 2003a).
 
Type strain:
 
Type sequences:LSU-AY387228;ITS-AY387132 
Link to previous version of yeast database:
 
26S Type to Centrality distance:100 
ITS minimum similarity (%) to Centrality:93.5532 
26S minimum similarity (%) to Centrality:90.1487 
ITS average similarity (%) between strains:92.803 
26S average similarity (%) between strains:93.8332 
ITS variance of similarity % between strains:17.6346 
26S variance of similarity % between strains:18.0644 
Number of ITS sequences accounted:17 
Number of 26S sequences accounted:32 
Genus:
 
Associated species:
 
Division:Basidiomycota 
Subdivision:Ustilaginomycotina 
Class:Exobasidiomycetes 
Subclass:Malasseziales 
Other strains:
 
ITS Remarks:

ITS Statistics for Malassezia globosa:
Average similarity between strains: 92.803%
Observed minimum similarity with central strain: 93.5532%
Similarity between type and central strain: 93.5532%
Statistics based on 17 sequences.

 
26S Remarks:

26S Statistics for Malassezia globosa:
Average similarity between strains: 93.8332%
Observed minimum similarity with central strain: 90.1487%
Similarity between type and central strain: 100%
Statistics based on 32 sequences.